Protesters in Surat Thani, Trang, Chumphon, Nakhon Ratchasima, Pattani, Yala, Phitsanulok, Phayao and Chon Buri provinces came to life after Prime Minister Yingluck Shinawatra announced the dissolution of the House of Representatives in the morning.
More than 30,000 whistleblowers gathered at Surat Thani's provincial hall and about 100 civil servants in the building stopped working and joined the protest.
In Trang, at least 10,000 demonstrators took to the streets to celebrate the House dissolution before settling at the provincial hall, awaiting the People's Democratic Reform Committee's next move.
Elsewhere, a traditional manora dance show with content mocking the government was being performed in front of 7,000-8,000 anti-government protestors led by Nopporn Usit near Chumphon's provincial hall. The state office was closed due to the rally.
About 5,000 whistleblowers surrounded the provincial hall in Pattani, demanding that Ms Yingluck step down as the head of the interim government. Another group of similar size surrounded Yala's provincial hall early Monday morning, preventing government officials entering the premises.
In Nakhon Ratchasima, at least 1,500 demonstrators led by Jakkrin Cherdchai paid a visit to the headquarters of the Provincial Police Region 3, the provincial hall and other state buildings.
Mr Jakkrin said the the acting premier's resignation would not suffice because they want the state to give power to the people in the form of a people's council.
Smaller numbers of anti-government protestors rallied in Phitsanulok, Phayao and Chon Buri provinces.
Security forces were deployed at the protest locations to ensure order.