The 54-year-old resident of Itabashi Ward in Tokyo is suspected of shooting his wife, also Chinese and believed to be in her 50s, at around 10.40pm Sunday at the hotel's cafe.
The suspect was seized by cafe employees and arrested at the scene by police on suspicion of attempted murder. The woman, who had been shot in the chest and jaw, was confirmed dead later.
The suspect has remained silent during police questioning, according to investigators.
None of the more than 10 customers at the cafe was injured.
Security camera footage showed the man and the woman quarreling at the cafe before the suspect began shooting.