The motorcyclists filled the road, causing congestion, with other motorists struck in a long line of cars extending for more than five kilometres.
Some members of the group who were stopped by police claimed they joined the trip to visit the scenic waterfall in Muak Lek district after a motorcyclist from Nonthaburi, named Durian Non, announced the rally on Facebook.
Saraburi highway police officer Snr Sgt Maj Wira Butpho said many of the motorcyclists did not have licences and rode without helmets. Many of the motorbikes had no vehicle registration plates.
Causing headaches for the police pursuing them, some of the motorcyclists also rode in the opposite direction of the prevailing traffic flow.
Many fled when police approached them and one rider crashed his motorcycle into an officer, said a Kaeng Khoi policeman.
He said the officer was injured but gave no further comment.
Pursuing officers said it was difficult to stop such a large number of motorcyclists, but they eventually managed to issue tickets to 50 of them, Kaeng Khoi police chief Pol Col Chakkrit Wiradet.
Durian Non posted details of the trip on his Facebook page on Aug 13.
It invited motorcyclists to join a rally to Chet Sao Noi waterfall.
He said they would start the journey from a petrol station at Pak Kret intersection in Nonthaburi and then meet other motorcyclists near Bangkok University on Vibhavadi Rangsit Road and drive to Saraburi together.
According to the itinerary, a photographer from a motorbike magazine would take pictures of the motorcyclists at the waterfall.
They would be accompanied by some hired "pretty" girls presenters.
One member of the rally said many people had come to ride their motorcycles in Saraburi to avoid police crackdowns in Bangkok.